SpermFreeze™ / SpermFreeze™ SSP
SpermFreeze™ and SpermFreeze™ SSP are ready-to-use cryopreservation media designed to protect human sperm through the freeze-thaw cycle. Both formulations combine glycerol, sucrose, and human serum albumin as cryoprotectants in a HEPES-buffered base, with SpermFreeze™ SSP offering a more concentrated option that accommodates up to three times the sperm volume per ml of medium. SpermFreeze™ is also validated for use with testicular biopsy material (TESE), making it a versatile choice across a range of andrological applications. CE-marked under MDR and FDA cleared.
Description
Sperm cryopreservation puts cells under significant stress, and the quality of the freezing medium is one of the few variables a lab can directly control. SpermFreeze™ is formulated to provide consistent, reliable cryoprotection for ejaculated sperm as well as sperm recovered from testicular biopsies (TESE) — a useful capability for clinics handling a range of male factor cases.
The standard SpermFreeze™ uses a 1:1 mixing ratio (0.7–1ml medium per 1ml sperm), while SpermFreeze™ SSP is a more concentrated formulation that requires only 1ml of medium for every 3ml of sperm. For labs processing higher sperm volumes or working under strict volume constraints — particularly relevant in TESE cases where sample volumes can vary — SSP offers a practical advantage without compromising cryoprotection.
Both media are HEPES-buffered and contain physiologic salts, glycine, glucose, and lactate alongside the cryoprotectant package: glycerol (15% in SpermFreeze™, 27% in SpermFreeze™ SSP), sucrose, and 4.0 g/l human serum albumin. The HSA is a medicinal substance derived from human blood plasma and contributes to membrane stabilization during the critical freezing and thawing phases.
Both products are ready to use straight from the vial — no preparation required. For labs looking to further standardize the process, FertiPro’s SpermFreeze Box accessory is available as a compatible add-on to improve consistency across freeze cycles. CE-marked under MDR (NB 2797), FDA cleared, and registered with Health Canada and Brazil.
